Hylaeus bituberculatus, or Hylaeus (Gnathoprosopoides) bituberculatus, is a species of bee in the family Colletidae and subfamily Hylaeinae. It is endemic to Australia. It was described by English entomologist Frederick Smith in 1879.

Distribution and habitat The species occurs in south-eastern Australia. Type localities include Melbourne, Sandringham, Gorae West, Kiata and Cann River in Victoria, as well as Launceston, Tasmania, and Blackheath, New South Wales.

Behaviour The adults are flying mellivores. Flowering plants visited by the bees include Angophora, Banksia, Boronia, Callistemon, Eucalyptus, Hypochaeris and Leptospermum species.

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