Zajíček (feminine: Zajíčková) is a Czech surname. It is a diminutive of the Czech word zajíc (meaning 'hare') and the name Zajíc. Notable people with the surname include:

Adam Zajíček (born 1993), Czech volleyball player Carl Wenzel Zajicek (1860–1923), Austrian painter Jan Zajíček (born 1977), Czech film director Jan Zajíček (ice hockey) (born 1951), Czech ice hockey player Jaroslav Zajíček (1920–2002), Czech cross-country skier Lubomír Zajíček (1946–2013), Czech volleyball player Pavel Zajíček (born 1951), Czech poet Phil Zajicek (born 1979), American road cyclist Šimon Zajíček (born 2001), Czech ice hockey player

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