DFE or DfE may refer to:

Organizations Department for the Economy, Northern Ireland Department for Education, UK DePatie–Freleng Enterprises, a defunct American animation studio

Science Difluoroescaline, a psychedelic drug Dilated fundus examination of the eye Distribution of fitness effects Dietary folate equivalent, bioavailability of folate 1,1-Difluoroethane

Technology Design-focused evaluation of educational quality Design for the Environment of a product or service DFE Ascender, an ultralight aircraft

Other Dragon Fli Empire, a Canadian hip hop group Dunfermline Town railway station, Scotland, National Rail station code The Duke of Edinburgh's Award, commonly referred to as 'DofE'