Ophioninae is a cosmopolitan subfamily of Ichneumonidae, comprising 33 genera and exhibiting high diversity in tropical regions. They are primarily koinobiont endoparasitoids of larval Lepidoptera, although at least one species parasitizes Scarabaeidae (Coleoptera). Pupae of Ophioninae are ovoid and feature a distinct central pale band, which is a characteristic trait of this subfamily. These insects are typically large, slender, and orange, with compressed and curved abdomens. They possess prominent ocelli and are primarily active during the night, often being attracted to light sources.

Genera Rousse et al. classified 13 of the genera of Ophioninae between 3 tribes, with the remaining genera unplaced to a tribe. The possibility of erecting a fourth tribe for the derived genera Hellwigia and Skiapus was also presented tentatively.

Tribe Enicospilini Dicamptus Enicospilus Hellwigiella Laticoleus

Tribe Ophionini Alophophion Afrophion Ophion - paraphyletic Rhopalophion Sclerophion Xylophion

Tribe Thyreodonini Dictyonotus Rhynchophion Thyreodon

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