De 24 uur van Daytona 1966 was de 5e editie van deze endurancerace. Het was tevens de eerste keer dat het een 24-uursrace was, nadat vorige edities over 3 uur of 2000 kilometer waren gehouden. De race werd verreden op 5 en 6 februari 1966 op de Daytona International Speedway nabij Daytona Beach, Florida.
De race werd gewonnen door de Shelby American Inc. #98 van Ken Miles en Lloyd Ruby, die allebei hun tweede achtereenvolgende Daytona-zege behaalden. De P2.0-klasse werd gewonnen door de Porsche System Engineering #15 van Hans Herrmann en Herbert Linge. De S2.0-klasse werd gewonnen door de Porsche System Engineering #16 van Gerhard Mitter en Joe Buzzetta. De GT+3.0-klasse werd gewonnen door de Roger Penske #12 van Dick Guldstrand, Ben Moore en George Wintersteen. De S+3.0-klasse werd gewonnen door de Ford Advanced Vehicles #86 van Peter Sutcliffe en Bob Grossman. De GT2.0-klasse werd gewonnen door de RBM Motors #18 van Jack Ryan, Lin Coleman en Bill Bencker. De S3.0-klasse werd gewonnen door de Jack Slottag #30 van Larry B. Perkins en Jack Slottag. De GT3.0-klasse werd gewonnen door de Cannon Auto #43 van Dana Kelder, Ara Dube en Red Wilson. De GT1.6-klasse werd gewonnen door de Pompano Sports Cars #84 van Ben Scott, Peter Flanagan en Roger Chastain. De S1.6-klasse werd gewonnen door de Autosport #80 van Donna Mae Mims, Janet Guthrie en Suzy Dietrich.
Race Winnaars in hun klasse zijn vetgedrukt.
Pos. Klasse No. Team Coureurs Chassis Ronden 1 P+2.0 98 Shelby American Inc. Ken Miles Lloyd Ruby Ford Mk.II 678 2 P+2.0 97 Shelby American Inc. Dan Gurney Jerry Grant Ford Mk.II 670 3 P+2.0 95 Holman & Moody Walt Hansgen Mark Donohue Ford Mk.II 669 4 P+2.0 21 North American Racing Team Pedro Rodríguez Mario Andretti Ferrari 365 P2 664 5 P+2.0 96 Shelby American Inc. Chris Amon Bruce McLaren Ford Mk.II 651 6 P2.0 15 Porsche System Engineering Hans Herrmann Herbert Linge Porsche 906 623 7 S2.0 16 Porsche System Engineering Gerhard Mitter Joe Buzzetta Porsche 904 GTS 612 8 S2.0 17 Porsche System Engineering Günter Klass Udo Schütz Porsche 904 GTS 610 9 P+2.0 22 North American Racing Team Jochen Rindt Bob Bondurant Ferrari 250 LM 591 10 S2.0 14 Brumos Porsche Florida Peter Gregg George Drolsom Porsche 904 GTS 589 11 S2.0 20 Sam Posey Sam Posey Jim Haynes Harry Theodoracopulos Porsche 904 GTS 577 12 GT+3.0 12 Roger Penske Dick Guldstrand Ben Moore George Wintersteen Chevrolet Corvette Sting Ray 575 13 P+2.0 23 Peter Clarke Mark Konig Peter Clarke Bob Hurt Ferrari 250 LM 574 14 S+3.0 86 Ford Advanced Vehicles Peter Sutcliffe Bob Grossman Ford GT40 571 15 P+2.0 27 David Piper David Piper Richard Attwood Ferrari 250 LM 556 16 GT2.0 18 RBM Motors Jack Ryan Lin Coleman Bill Bencker Porsche 911 548 17 S+3.0 92 Essex Wire Corporation Peter Revson Masten Gregory Ed Lowther Ford GT40 531 18 GT+3.0 90 Michael Reina Don Kearney Michael Reina Ford Mustang 527 19 S3.0 30 Jack Slottag Larry B. Perkins Jack Slottag Ferrari 250 GTO 526 20 GT+3.0 67 Cannon Auto George Cornelius Dick Boo Bob Brown Chevrolet Corvette Sting Ray 501 21 S2.0 56 William Martin Ike Maxwell William Martin Volvo P1800 496 22 P2.0 41 E. P. Drescher Hugh Kleinpeter Harry Fry George Parsons Triumph LM 490 23 GT3.0 43 Cannon Auto Dana Kelder Ara Dube Red Wilson Triumph TR4A 487 24 GT3.0 45 John Kingham Herb Byrne Milo Vega Triumph TR4A 482 25 GT1.6 84 Pompano Sports Cars Ben Scott Peter Flanagan Roger Chastain Alfa Romeo Giulia Sprint 474 26 GT+3.0 54 Ray Stoutenburg Ray Stoutenburg Gene Jones Larry Isley Plymouth Barracuda 466 27 P+2.0 75 McMillan Ring Free Oil Co. Art Riley Russ MacGrotty Yenko Stinger 448 28 GT3.0 40 Genser Forman George Waltman Art Swanson Nick Cone Triumph TR4 432 29 P2.0 7 John Olson John Bolander Bob Winkelmann John Olson Ford Cortina 416 30 S2.0 78 Autosport Rosemary Smith Smokey Drolet John Olson Sunbeam Alpine 416 31 S2.0 73 Fred Opert William McKemie Terry Petmecky Fred Opert Elva Courier Mk.IV 391 32 S1.6 80 Autosport Donna Mae Mims Janet Guthrie Suzy Dietrich Sunbeam Alpine 389 NC P2.0 79 Autosport Paul Richards Ray Cuomo Austin-Healey Sprite 491 DNF S+3.0 91 Essex Wire Corporation Dick Thompson Skip Scott Peter Revson Ford GT40 500 DNF P+2.0 32 North American Racing Team George Follmer Don Wester Paul Hawkins Ferrari 250 LM 428 DNF GT2.0 44 Kenneth Chambliss Ernie Croucher Walter Glenn Bill Eve MGB 426 DNF S+3.0 29 Epstein Enterprises Ltd. Paul Hawkins Jackie Epstein Ferrari 250 LM 425 DNF S+3.0 94 Ralph Noseda Ralph Noseda Grant Clark Bob Thorpe Harry Heuer Shelby Cobra 419 DNF GT1.6 55 J. Randall Al Weaver John Fraim Chet Freeman Lotus Elan 383 DNF S+3.0 93 Scuderia Bear Harold Keck Oscar Koveleski Ed Lowther Shelby Cobra 371 DNF P+2.0 87 Holman & Moody Richie Ginther Ronnie Bucknum Ford Mk.II 329 DNF S+3.0 89 Dan Gerber Dan Gerber Bob Johnson Peter Lerch Shelby Cobra 328 DNF P+2.0 65 Chaparral Cars Inc. Jo Bonnier Phil Hill Chaparral 2D 318 DNF S1.6 36 Cannon Auto Wilbur Pickett Bill Bean Alfa Romeo Giulia Zagato 278 DNF GT3.0 42 John Addison Ken Hughes Fred Salo John Addison Triumph TR4 244 DNF S2.0 59 Art Riley Don Yenko John Forte Art Riley Volvo P1800 216 DNF P+2.0 31 John Fulp John Fulp Bill Rutan Bruce Jennings Ferrari 330 P 193 DNF S+3.0 88 William Wonder William Wonder Herb Wetanson Ford GT40 178 DNF P+2.0 25 Ecurie Francorchamps Lucien Bianchi Gérard Langlois van Ophem Jean Blaton Ferrari 365 P2 171 DNF GT+3.0 99 Joe Treadwell Roger West Dick Macon Ford Mustang 168 DNF P+2.0 24 Drummond Racing Organisation Innes Ireland Mike Hailwood George Drummond Ferrari 250 LM 90 DNF GT1.6 19 David McClain Duncan Forlong Leland Dieas David McClain Porsche 356B Super 90 80 DNF P+2.0 26 Ecurie Francorchamps Léon Dernier Jacky Ickx Ferrari 250 LM 80 DNF P+2.0 74 Queen City Rambler Larry Hess Tommy Hess Rambler Marlin 80 DNF S2.0 47 David Lane David Lane Don Sesslar Porsche 904 GTS 74 DNF GT+3.0 77 Space Science John Bentley John Hill Sunbeam Tiger 71 DNF GT3.0 5 Robert Harper Spurgeon May Bobby Allison Chevrolet Corvair 63 DNF P+2.0 28 Team Chamaco Collect Denny Hulme Vic Wilson Ferrari 250 LM 53 DNF GT+3.0 12 Jaguar of Florida Dave Hull Herb Byrne Bob Kingham Jaguar E-Type 41 DNS GT1.6 85 Anatoly Arutunoff Bill Clark Bryan Crow Anatoly Arutunoff Alfa Romeo Giulia Sprint 0
1966 Daytona